Skip to Content

Bioanalyst Zone Interview (free login required)

novel single-sensor multiplexed marker assay – a step towards simplified disease management and detection? Author:Natalie Harrison, Future Science Group Researchers from the Biodesign Institute at ArizonaState University (ASU), USA engineer a sensor capable of simultaneously measuring multiple analytes in a label-free and rapid manner The group, led by Dr Jeffrey La Belle, Assistant Research Professor at ASU, were able to measure the interaction between ultralow levels of analyte (the typical inflammatory markers, interleukin-12 and TNF-α) and molecular recognition elements by electrochemical impedance spectroscopy and quantification. Asked why this work is so important, La Belle stipulated that for many diseases, relying on a single biomarker is inefficient and not always most accurate, highlighting prostate specific antigen (PSA) for prostate cancer and glucose monitoring for diabetes as examples. Elaborating on PSA: “The clinical community acknowledges it is a viable marker, but many results still need cross confirmation from a digital rectal exam (DRE) to be accurate or sensitive enough to diagnose cancer“. In the case lipitor dosage of diabetes, for the past 30 years, glucose has remained the only monitored biomarker. In addition to noncompliance when it comes to self-monitoring blood glucose, quarterly tests like glycosylated hemoglobin, HbA1c, are only adhered to, on average, once a year. “This noncompliance has led to direct and indirect costs of diabetes generating an estimated $500 billion a year in additional costs to US health care, which is not sustainable and needs to be lowered- this is up from $300 billion in 2009 and $174 billion in 2007. A better test, providing more metabolic, immunological and inflammation type data could help,” remarked La Belle. The ASU professor assigned the task of putting all the targets on the same sensor, as well as the sheer novelty of the work, as the ceftin biggest hurdles his team have faced. He continued: “instrumentation, that’s another issue- we needed biologists, endocrinologists, engineers – electrical, computer, biomedical, and that’s just to figure this all out. Luckily at ASU, we have a great student population to draw from and support from the School of Biological and Health Systems Engineering’s Biomedical Engineering program”. The next steps for the researchers will involve developing or adapting models to understand, why, electrochemically, this technique works and makes sense. As La Belle points out: “data is one thing, complete understanding is another”. There remains a lot of work to be done in the field of identifying markers for particular diseases; especially in animal studies. La Belle speculates: “sometimes when you determine one frequency of one biomarker and then look at another biomarker of interest, both can be very close to one another and therefore are hard to differentiate”, as was the case for the inflammatory markers, which demonstrated considerable overlap (Figures 1 & 2). “Hence our tuning could be used to move one frequency away from the other and

no prescription birth controlpermethrin creamcitalopram without prescriptionviagra online order pricecialis user reviews propecia side effects for menfree cialis pillsviagra side effectviagra paypalpagemexican pharmacy no prescriptionviagra online canadaonline prescription cialisbuy trazodonewellbutrin sr reviewsviagra online storeantibiotics on lineexelon tabletsgogeneric cialis 10mghow to purchase viagrano prescription birth control sudetsol.beviagra australialevitra tabletscialis mail orderrevatio 20 mgcheap antibioticsonline drugstore without prescriptioncompare prices cialisviagra non prescription prescriptions sudetsol.be costlow price viagrahow much does cialis costed medicines parki-razvlecheniy.orgonline drugstore without prescriptionmexican pharmacy online no prescription canadian pharmaciesfree cialis offerbuy thyroxine100 mg cialis discount cardhow to buy cialis parki-razvlecheniy.orgcialis online buy online dosagesphenergan suppositorylow cost cialisbuying cialiscialis levitra meds online no prescription prescription drugs without rxviagra menonline prescription drugsviagra sildenafil depression medicineviagra sildenafil citratebuy nolvadex without prescription viagraovernight cialisbuy tretinoin creamovernight viagra deliverycanada pharmacy online no prescriptionpropecia without prescriptionsbest generic viagraorder viagra online overnightaccutane pillsviagra on craigslistpharmacy drugseli lilly cialischeap pharm cialis indiacanadian viagra pharmacyxenical 120mglinkletrozole cost pharmacy viagrafemale viagra uknon prescription viagra canadacialis dosage side effectsviagra online reviewscanadian pharmacy mallsildenafil citrate onlinecialis 5mg tabletsbuy viagra without prescription cialis onlinez pak 3 dayovernight viagra deliverypharmacy escrow refillsmedicare viagralevaquin lawsuitweight loss with pcosclick hereerection pills at walmartorder synthroidprice for cialis cialisprescription drugs india storecialis trialcialis effectiveness viagra australiabuy prozac online25 mg viagraview sitegeneric cialis reviewpfizer viagra couponszoloft for saleinternational pharmacy no prescriptioncialis canadian pharmacy pillsgeneric viagra reviewscialis free trailcialis online prescriptionno prescription online pharmacycialis c20cialis prescription onlinedrugs that cause edmetformin over the counter walgreens pottstownchryslerservice.comonline pharmacy without a prescriptionside effects from viagra saipore.comviagra for femalescialis in indiabuy antibiotics without prescriptiononline prescription viagramost trusted online pharmacy for daily use review genericorder cialis online canadapurchase cialis online cheapbuy levitra online generic 1mgbuy viagra in australia brand cialis onlinecanadian viagra no prescriptionwhere can i buy tretinoinrx pill shopprednisone 20 mgbuy viagra australia hydrochlorothiazide cialis online pottstownchryslerservice.com2.5 mg cialiscialis effect viagra overnightnewhealthymanbuy norvasc onlinecialis samples onlinecialis australiaviagra prescription costviagra meneffects of viagra on menlevitra cialisbrand name cialised drugs over the counterviagra by mailsildenafil ventolin without prescriptioned treatment reviewuse of viagrageneric abilifycheap online cheap viagra 100mgonline generic cialispurchase drugs onlinedoxycycline shortageno prescription online pharmacy 10 mgviagra sublingualelocon creamcialis pillonline viagra sales rec-yotsukaidou.comcialis brand name online online australia paypalside effects of prednisone withdrawalorder cheap cialisbuy propeciacialis pharmacy of edno prescription drugsbuy amoxicillinpurchase retin a onlinebuy tretinoin creamviagra generic datecialis generic onlineview site to buy cialisviagra genericsclavamox for humansthe blue pillprescription free viagratrial viagraherbal viagracialis side effectcheapest viagra onlineclaravis onlineviagra side effects for mengnc erectionwomen s viagra pricesherbal viagra alternativecialis 2.5 mgbuy tadacip 20 mgbest ed pillcost of cialised medicine onlinebuy viagra international pharmacy no prescriptioncialis free trial offergeneric viagra 50mgbuy viagra no prescriptiondoxycycline canada for sale no prescriptioncanadian pharmacydoxycycline canada inhalersbuy bactrim online no prescriptionviagra costviagra professional review 20mged medicine online24h pharmacyclaravis online sudetsol.bebuy amoxicillin without prescriptionno prescription pharmacyover the counter antibioticsbuy levitra robaxin get you highcheapest generic cialismuse ed 20mg priceover the counter permethrin creambirth control no prescriptionside effects of viagra on menonline prescriptionspfizer viagra onlineed treatment reviewprintable cialis couponscanadian pharmacy without prescriptioncialis canadiandrugs for depression and anxietycheap drugs onlinemeds from mexicosubstitute viagra pills rica pharmacy online mg online pharmacy indiaturkish pharmacyprednisone packwhen does accutane start workingfree viagra sample pack by mail overseas pharmacybuy cialis 20mg mexico pharmacy websiteamaryl for diabetesside effects of viagra cialis onlinebest overseas pharmacy pfizer viagracialis once dailycialis free offercanadian viagraclickonline prescription viagraazithromycin for sale online 200 mgcialis sales“click here”natural viagra substitutespill identifier with picturesviagra malaysiacanadian pharmacy paypal accepted cialis dosageno prescription needed pharmacycheap viagra free shipping

put all biomarker recognition elements onto a single device” (Figure 3). He went on to speculate that development of instrumentation and signal processing, currently under development, are now required (Figure 4). With the ability to multiplex targets and still use an assay that takes under 90 seconds per sample, the wider implications of this work extend all the way to high-throughput screening. Concluding with the question of future technological developments, La Belle remarked: “we are starting to partner with collaborators at ASU and in industry to obtain the right instrumentation for the studies. We are also collaborating with the Mayo Clinic in Scottsdale to have clinical experts and studies to be preformed when we are ready for that stage, we are still early on”. Source: La Belle J, Demirok U, Patel D, Cook C. Development of a novel single sensor multiplexed marker assay. Analyst, 2011, 136, 1496-1501

Leave a Reply